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Sherman Lab

Brief Biography

Dr. Sherman is Professor of Paediatrics, Microbiology and Dentistry at The Hospital for Sick Children, University of Toronto where he has been a faculty member since 1984. Dr. Sherman is the recipient of a Tier 1 Canada Research Chair in Gastrointestinal Diseases (2001-2008). His research program is funded by support provided by the Canadian Institutes of Health Research (1985 – present) and the Crohn’s and Colitis Foundation of Canada (1986-1991, 1997-2000, 2001-2004, 2007-2010).

Research Interests

Research interests focus on gastrointestinal epithelial cell responses to bacterial pathogens and their products.

• Shigatoxin - producing Escherichia coli
• Paediatric inflammatory bowel disease
• Probiotics
• Signal transduction

External Funding

• Canadian Association of Gastroenterology
• Canadian Institutes of Health Research
• Crohn’s and Colitis Foundation of Canada
• Institute Rosell-Lallemand Inc.

Canadian Institutes of Health Research (CIHR) Team Grant, 2007-2012.
"Immune regulation and biomarker development in pediatric and adult onset human autoimmune diseases" Amit Bar-Or, Principal Investigator.

Crohn's and Colitis Foundation of Canada. "Fay Shapiro Cutler Grant-In-Aid of Research" 2007-2010.
"Role of probiotics in the management of experimental inflammatory bowel diesase"

Canadian Institutes of Health Research (CIHR), Training Grant 2002-2008.
"Cell signalling in mucosal inflammation and pain ", Richard Ellen, Principal Investigator

Canadian Institutes of Health Research (CIHR), 2008-2013
Epithelial cell signalling responses to attaching - effacing pathogens

Achievements

1. Associate Director, Institute of Medical Science, 2007 - 2011
2. Co-director CIHR training grant, Cell signalling in mucosal inflammation and pain, 2006 - 2008
3. Institute of Medical Science, Mel Silverman Mentorship Award, 2007
4. AHFMR Visiting Scientist to Alberta Award, 2003

Publications

Wine E, Gareau MG, Johnson-Henry K, Sherman PM. Strain-specific probiotic (Lactobacillus helveticus) inhibition of Campylobacter jejuni invasion of human intestinal epithelial cells.  FEMS Microbiol Lett. 2009 ;300:146-152..

Wine E, Ossa JC, Gray-Owen SD, Sherman PM. Adherent-invasive Escherichia coli, strain LF82 disrupts apical junctional complexes in polarized epithelia. BMC Microbiol.  2009;9:180-190.

Jandu N, Ho NK, Donato KA, Karmali MA, Mascarenhas M, Duffy SP, Tailor C, Sherman PM. Enterohemorrhagic Escherichia coli O157:H7 gene expression profiling in response to growth in the presence of host epithelia.  PLoS One. 2009;4(3):e4889.

Sherman PM, Ossa JC, Johnson-Henry K. Unraveling mechanisms of action of probiotics.  Nutr Clin Pract. 2009 Feb-Mar;24(1):10-4.

Jandu N, Zeng ZJ, Johnson-Henry KC, Sherman PM. Probiotics prevent enterohaemorrhagic Escherichia coli O157 : H7-mediated inhibition of interferon-{gamma}-induced tyrosine phosphorylation of STAT-1. Microbiology 2009 155: 531-540.

Sherman PM, Wine E. Emerging intestinal infections. Gastroenterology and Hepatology Annual Review 2006;1:50-4.

Wine E, Chan VL, Sherman PM. Campylobacter jejuni mediated disruption of polarized epithelial monolayers is cell-type specific, time dependent, and correlates with bacterial invasion. Pediatr Res. 2008 Dec;64(6):599-604.

Donato KA, Zareie M, Jassem AN, Jandu N, Alingary N, Chan Carusone S, Johnson-Henry KC, Sherman PM. Escherichia albertii and Hafnia alvei are candidate enteric pathogens with divergent effects on intercellular tight junctions. Microb Pathog. 2008 Nov-Dec;45(5-6):377-85.

Johnson-Henry KC, Sherman PM. Exploring frontiers of new probiotic and pharmabiotic therapies. Nutrafoods 2008;7(2/3): pp 27-36.

Johnson-Henry KC, Donato KA, Shen-Tu G, Gordanpour M, Sherman PM. Lactobacillus rhamnosus strain GG prevents enterohemorrhagic Escherichia coli O157:H7-induced changes in epithelial barrier function. Infect Immun. 2008;76:1340-8.

Johnson-Henry K, Hagen K, Gordonpour M, Tompkins T, Sherman P. Surface-layer protein extracts from Lactobacillus helveticus inhibit enterohaemorrhagic Escherichia coli O157:H7 adhesion to epithelial cells. Cellular Microbiology, 9:356-367, 2007.

Jandu N, Shen S, Wickham M, Prajapti R, Finlay B, Karmali M, Sherman P. Multiple seropathotypes of verotoxin-producing Escherichia coli disrupt interferon-gamma-induced tyrosine phosphorylation of signal transducer and activation of transcription-1. Microbial Pathogenesis, 42:62-71, 2007.

Skinn A, Vergnolle N, Cellars L, Sherman P, MacNaughton W. Combined challenge of mice with Citrobacter rodentium and ionizing radiation promotes bacterial translocation. International Journal of Radiation Biology, 83:375-382, 2007.

Gareau M, Jury J, MacQueen G, Sherman P, Perdue M. Probiotic treatment of rat pups normalizes corticosterone release and ameliorates colonic dysfunction induced by maternal separation. Gut Published Online First: 5 March 2007. doi:10.1136/gut.2006.117176.

Muise A, Walters T, Wine E, Griffiths A, Duerr R, Turner D, Regueiro M, Ngan B-Y, Sherman P, Silverberg M, Rotin D. Protein tyrosine phosphatase sigma is associated with ulcerative colitis. Curr Biol. 2007 Jul 3; [Epub ahead of print] PMID: 17614280

Nazli A, Wang A, Steen O, Prescott D, Lu J, Perdue M, Söderholm J, Sherman P, McKay D. Enterocyte cytoskeleton changes are crucial for enhanced translocation of nonpathogenic Escherichia coli across metabolically stressed gut epithelia. Infection and Immunity, 74:192-201, 2006.

Yang PC, Jury J, Söderholm J, Sherman P, McKay D, Perdue M. Chronic psychological stress in rats induces intestinal sensitization to luminal antigens. American Journal of Pathology, 168:104-114, 2006.

Jandu M, Ceponis P, Kato S, Riff J, McKay D, Sherman P. Conditioned medium from enterohemorrhagic Escherichia coli-infected T84 cells inhibits signal transducer and activation of transcription-1 activation by gamma interferon. Infection and Immunity, 74:1809-1818, 2006.

O'Hara J, Skinn A, MacNaughton W, Sherman P, Sharkey K. Consequences of Citrobacter rodentium infection on enteroendocrine cells and the enteric nervous system in the mouse colon. Cellular Microbiology, 8:646-660, 2006.

Zareie M, Johnson-Henry K, Jury J, Yang P, Ngan B, McKay D, Söderholm J, Perdue M, Sherman P. Probiotics prevent bacterial translocation and improve intestinal barrier function in rats following chronic psychological stress. Gut, 55: 1553-1560, 2006.

Skinn A, Vergnolle N, Zamuner S, Wallace J, Cellars L, MacNaughton W, Sherman P. Citrobacter rodentium causes iNOS-independent intestinal epithelial dysfunction in mice. Canadian Journal of Physiology and Pharmacology, 84:1301-1312, 2006.

Ceponis P, Riff J, Sherman P. Epithelial cell signaling response to enterohemorrhagic Escherichia coli infection. Memorias Institute Oswaldo Cruz, 100 (Suppl. 1):199-203, 2005.

Lin F, Sabri M, Alirezaie J, Li D, Sherman P. Development of a nanoparticle-labeled microfluidic immunoassay for the detection of pathogenic microorganisms. Clinical and Diagnostic Laboratory Immunology, 12:418-425, 2005.

Hansen K, Sherman P, Cellars L, Andrade-Gordon P, Pan Z, Baruch A, Wallace J, Hollenberg M, Vergnolle N. A major role for proteolytic activity and proteinase-activated receptor-2 in the pathogenesis of infectious colitis. Proceedings of the National Academy of Sciences (USA), 102:8363-8368, 2005.

Johnson-Henry K, Nadjafi M, Avitzur Y, Mitchell D, Ngan B-Y, Galindo-Mata E, Jones N, Sherman P. Amelioration of the effects of Citrobacter rodentium infection in mice by pretreatment with probiotics. Journal of Infectious Diseases, 191:2106-2117, 2005.

Gao Y, Lin F, Hu G, Sherman P, Li D. Development of a novel electrokinetically-driven microfluidic immunoassay for the detection of Helicobacter pylori. Analytica Chimica Acta, 543:109-116, 2005.

Sherman P, Johnson-Henry K, Yeung H, Ngo P, Goulet J, Tompkins T. Probiotics reduce enterohemorrhagic Escherichia coli O157:H7 and enteropathogenic E. coli O127:H6-induced changes in polarized T84 epithelial cell monolayers by reducing bacterial adhesion and cytoskeletal rearrangements. Infection and Immunity, 73:5183-5188, 2005

Johnson-Henry K, Riff J, Gordanpour M, Sherman P. Probiotics in models of gastrointestinal bacterial infections. Nutrafoods, 4:29-36, 2005.

Riff J, Callaghan J, Sherman P. Cholesterol-enriched membrane microdomains are required for inducing host cell cytoskeleton rearrangements in response to attaching-effacing Escherichia coli. Infection and Immunity, 73:7113-7125, 2005.

Hu G, Gao Y, Sherman P, Li D. A microfluidic chip for heterogeneous immunoassay using electrokinetical control. Microfluidics and Nanofluidics, 1:346-355, 2005.

Zareie M, Riff J, Donato K, McKay D, Perdue M, Söderholm J, Karmali M, Cohen M, Hawkins J, Sherman P. Novel effects of the prototype translocating Escherichia coli, strain C25 on intestinal epithelial structure and barrier function. Cellular Microbiology, 7:1782-1797, 2005.

Gao Y, Hu G, Lin F, Sherman P, Li D. An electrokinetically-controlled immunoassay for simultaneous detection of multiple microbial antigens. Biomedical Microdevices, 7:301-312, 2005.

Söderholm JD, Streutker C, Yang P-C, Paterson C, Singh PK, McKay DM, Sherman PM, Croitoru K, Perdue MH. Increased epithelial uptake of protein antigens in the ileum of Crohn’s disease mediated by tumour necrosis factor-α, Gut, 53:1817-1824, 2004.

Day A, Su B, Ceponis P, Jones N, Yau E, Sieveking D, Sherman P. Helicobacter pylori infection induces interleukin-18 production in gastric epithelial (AGS) cells. Digestive Diseases and Sciences, 49:1830-1835, 2004.

Lin F, Sabri M, Erickson D, Alirezaie J, Li D, Sherman P. Development of a novel microfluidic immunoassay for the detection of Helicobacter pylori infection. The Analyst, 129:823-828, 2004.

Johnson-Henry K, Mitchell D, Avitzur Y, Galindo-Mata E, Jones N, Sherman P. Probiotics reduce bacterial colonization and gastric inflammation in Helicobacter pylori-infected mice. Digestive Diseases and Sciences, 49:1095-1102, 2004.

Lin F, Sherman P, Li D. Development of a novel hand-held immunoassay for the detection of enterohemorrhagic Escherichia coli O157:H7. Biomedical Microdevices, 6:125-130, 2004.

Nazli A, Yang P-C, Jury J, Howe K, Watson J, Söderholm J, Sherman P, Perdue M, McKay D. Epithelia under metabolic stress perceive commensal bacteria as a threat. American Journal of Pathology, 164:947-957, 2004.

Sherman P, Mitchell D, Cutz E. Neonatal enteropathies: defining the causes of protracted diarrhea of infancy. Journal of Pediatric Gastroenterology and Nutrition, 38:16-26, 2004.

Mitchell D, Huynh H, Ceponis P, Jones N, Sherman P. Helicobacter pylori disrupts Stat1-mediated interferon gamma-induced signal transduction in epithelial cells. Infection and Immunity, 72:537-545, 2004.

Ceponis P, McKay D, Ching J, Perreira P, Sherman P. Enterohemorrhagic Escherichia coli O157:H7 disrupts Stat 1-mediated gamma interferon signal transduction in epithelial cells. Infection and Immunity, 71:1396-1404, 2003.

Jin S, Song Y, Emil A, Sherman P, Chan V. JlpA of Campylobacter jejuni interacts with surface-exposed heat shock protein 90 leading to activation of NF-·B and p38 MAP kinase in epithelial cells. Cellular Microbiology, 5:165-174, 2003.

Su B, Ceponis P, Lebel S, Huynh H, Sherman P. Helicobacter pylori activates Toll-like receptor 4 expression in gastrointestinal epithelial cells. Infection and Immunity, 71:3496-3502, 2003.

Su B, Ceponis P, Sherman P. Cytoskeletal rearrangements in gastric epithelial cells in response to Helicobacter pylori infection: Journal of Medical Microbiology, 52:861-867, 2003.

Mooney A, Byrne C, Clyne M, Johnson-Henry K, Sherman P, Bourke B. Invasion of human epithelial cells by Campylobacter upsaliensis. Celllular Microbiology, 6:835-847, 2003.

Jones N, Day A, Jennings H, Shannon P, Galindo-Mata E, Sherman P. Enhanced disease severity in Helicobacter pylori-infected mice deficient in Fas signaling. Infection and Immunity, 70:2591-2597, 2002.

Ching J, Jones N, Ceponis P, Karmali M, Sherman P. Escherichia coli Shiga-like toxins induce apoptosis and cleavage of poly (ADP-ribose) polymerase via in vitro activation of caspases. Infection and Immunity, 70:4669-4677, 2002.

Söderholm J, Yang P, Ceponis P, Vohra A, Riddell R, Sherman P, Perdue M. Chronic stress induces mast cell dependent bacterial adherence and initiates mucosal inflammation in rat intestine. Gastroenterology, 123:1099-1108, 2002.