SIDNET shRNA Archive
shRNA Collection:
SIDNET houses both human and mouse pGIPZ lentiviral shRNAmir-GFP libraries developed by Open Biosystems in collaboration with Dr. Greg Hannon (CSHL) and Dr. Steve Elledge (Harvard), offering access to genome wide RNAi resources. The Human library contains >145,000 constructs and the Mouse Library > 110,000 constructs. These libraries combine the design advantages of microRNA-adapted shRNA with the pGIPZ vector to create a powerful RNAi trigger capable of producing RNAi in a variety of cell types. TurboGFP and shRNAmir are part of a bicistronic transcript allowing the visual marking of shRNAmir expressing cells. Additionally SIDNET can package pGIPZ shRNA lentiviral particles for transduction into difficult cell lines, including primary and non-dividing cells.
Distribution of pGIPZ shRNA is limited to SickKids Investigators
pGIPZ shRNA constructs are available at significant savings to all SickKids investigators. Please see the PRICE LIST 
for details.
From knockdown of single genes to complete pathways SIDNET’s shRNA screening services will accelerate the pace of discovery and augment your research.
To access the complete shRNA archive please log into the SIDNET/Openbiosystems portal and follow the Ordering Instructions. A partner password required. Please contact Christopher Fladd for details.
Available pGIPZ gene families and pathways defined by Gene Ontology:
pGIPZ shRNAmir gene families provide the advantages of shRNAmir design combined with the convenience of Gene ontology (GO) annotated collections targeting all genes in a particular gene family or biological pathway.
The arrayed sets contain shRNAmir from the human and mouse GIPZ lentiviral shRNAmir libraries that target all genes falling under specific GO terms. These collections will greatly simplify pathway analysis, target identification and validation.
| Human pGIZP Gene Families | Mouse pGIPZ Gene Families |
| Angiogenesis | Angiogenesis |
| Apoptosis | Apoptosis |
| Cell Cycle | Cell Cycle |
| GPCR | GPCR |
| Kinase | Kinase |
| Phosphatase | Phosphatase |
| Transcription Factor | Transcription Factor |
| Ubiquitin | Ubiquitin |
| Ubiqutin Ligase | Ubiqutin Ligase |
Detailed information and instructions can be found in the pGIPZ Manual, Arrest-In Manual, Lentiviral shRNAmir TechNote
SIDNET pGIPZ Limited Use License Agreement