Vector information
Please note: the following links contain an assortment of file types and formats.
Human ORFeome Clones:
Gateway Entry Vector Information:
Mammalian Gene Collection (MGC) Clones:
- MGC Clone Vector Information and Growth Instructions
- IRAT Plate Vector List
- IRAU Plate Vector List
- IRCM Plate Vector List
- MGC Plasmid Details
- MGC IRAT, IRAU and IRCM Plates available at SIDNET
SIDNET Destination Vectors Summary:
- SIDNET Destination Vectors (in PDF)
- SIDNET Destination Vectors (in Excel)
Vector Information:
High-level E. coli Expression Vectors:
- Native Expression (no tag)
- pDEST 14 Sequence, pDEST 14 Map, pDEST 14 Polylinker
- N-terminal GST
- pDEST 15 Sequence, pDEST 15 Map, pDEST 15 Polylinker
- N-terminal 6XHis
- pDEST 17 Sequence, pDEST 17 Map, pDEST 17 Polylinker
- N-terminal 6XHis-Lumio Tag-TEV recognition site
- pEXP3-DEST Sequence, pEXP3-DEST Map, pEXP3-DEST Polylinker
[In pDEST 14, 15 17 and pEXP3-DEST expression of the gene of interest is controlled by the T7 promoter.
Bacterial strains that express the T7 RNA polymerase are required for protein expression]
Mammalian Constitutive Expression Vectors:
- C-terminal V5 epitope-6XHis
- pcDNA-DEST40 Sequence, pcDNA-DEST40 Map, pcDNA-DEST40 Polylinker
- C-terminal Cycle 3 GFP
- pcDNA-DEST47 Sequence, pcDNA-DEST47 Map, pcDNA-DEST47 Polylinker
- N-terminal Cycle 3 GFP
- pcDNA-DEST53 Sequence, pcDNA-DEST53 Map, pcDNA-DEST53 Polylinker
- N-terminal V5 Epitope-TEV recognition site
- pcDNA3.1 nV5-DEST Sequence, pcDNA3.1 nV5-DEST Map, pcDNA3.1 nV5-DEST Polylinker
- C-terminal V5 Epitope
- pcDNA3.2-DEST Sequence, pcDNA3.2-DEST Map, pcDNA3.2-DEST Polylinker
- N-terminal 6XHis
- pDEST 26 Sequence, pDEST 26 Map, pDEST 26 Polylinker
- N-terminal GST
- pDEST 27 Sequence, pDEST 27 Map, pDEST 27 Polylinker
- N-terminal HA Epitope
- pcDNA3.1/nHA-DEST Map and Polylinker
- N-terminal Myc Epitope
- pcDNA3.1/nMyc-DEST Map and Polylinker
- N-terminal Flag Epitope
- pcDNA3.1/nFlag-DEST Map and Polylinker
- C-terminal Myc Epitope
- pcDNA3.2/cMyc-DEST Map and Polylinker
- N-terminal mOrange Fluorescent Protein Fusion
- pcDNA3.1 mOrange
- N-terminal mStrawberry Fluorescent Protein Fusion
- pcDNA3.1 mStrawberry
- N-terminal mCherry Fluorescent Protein Fusion
- pcDNA3.1 mCherry
- C-terminal Emerald GFP Fluorescent Protein Fusion
- pcDNA6.2 C-EmGFP-DEST Sequence, pcDNA6.2 C-EmGFP-DEST Map, pcDNA6.2 C-EmGFP-DEST Polylinker
- N-terminal Emerald GFP Fluorescent Protein Fusion
- pcDNA6.2 N-EmGFP-DEST Sequence, pcDNA6.2 N-EmGFP-DEST Map, pcDNA6.2 N-EmGFP-DEST Polylinker
Mammalian Inducible Expression Vectors:
- Tetracycline Operator with N-terminal 6XHis
- pT-REx-DEST31 Sequence, pT-REx-DEST31 Map, pT-REx-DEST31 Polylinker
Insect Constitutive Expression Vectors:
- Metallothionein promoter with C-terminal V5 Epitope and 6XHis
- pMT-DEST48 Sequence, pMT-DEST48 Map, pMT-DEST48 Polylinker
Please note: Unfortunately, SIDNET can NOT distribute "Empty" vectors!
Control Expression Western Blots for Various Gateway Expression Vectors: